Binding of Ligands to the Inorganic Pyrophosphatase of Bakers' Yeast
نویسندگان
چکیده
منابع مشابه
THE CALCIUM BINDING SITES OF THE BAKERS' YEAST TRANSKETOLASE
The calcium binding sites of Bakers' Yeast Transketolase (TK) was elucidated by estimating the pKa values of the functional groups that bind to calcium. These pKa's were found to be 6.25 and 7.2 relating to the pKa's of the two immidazol moieties of histidine residues on the enzyme. The rate of the binding of calcium to the enzyme was obtained separately as a function of pH. Maximum values ...
متن کاملPurification of yeast inorganic pyrophosphatase.
An enzyme which hydrolyzes inorganic pyrophosphate (PP) to orthophosphate (ortho P) was demonstrated by Lohmann (1) in muscle. Bauer (2) found that autolysates of slowly dried bottom yeast were a rich source of inorganic pyrophosphatase and were free of adenosinetriphosphatase (ATPase) activity. An important contribution was made by Bailey and Webb (3) who accomplished an extensive purification...
متن کاملYeast Inorganic Pyrophosphatase
The kinetics of the Mg2+ activation of crystalline yeast inorganic pyrophosphatase have been thoroughly investigated from pH 7.40 to pH 9.05 using a sensitive isotope assay in order to determine the role of the divalent metal ion activator in the reaction. A computer program has been devised for calculating the concentration of each of the various components of the complex equilibrium involving...
متن کاملYeast Inorganic Pyrophosphatase
The magnesium ion-dependent hydrolysis of pyrophosphate catalyzed by yeast inorganic pyrophosphatase is markedly inhibited by calcium ion. The kinetics of inhibition have been measured and analyzed in terms of a model involving strong interactions between CaPPi and two metalcomplexed forms of the enzyme, EMg and ECa. Inhibition constants for these interactions have been estimated by nonlinear r...
متن کاملthe calcium binding sites of the bakers' yeast transketolase
the calcium binding sites of bakers' yeast transketolase (tk) was elucidated by estimating the pka values of the functional groups that bind to calcium. these pka's were found to be 6.25 and 7.2 relating to the pka's of the two immidazol moieties of histidine residues on the enzyme. the rate of the binding of calcium to the enzyme was obtained separately as a function of ph. maxi...
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ژورنال
عنوان ژورنال: European Journal of Biochemistry
سال: 1973
ISSN: 0014-2956,1432-1033
DOI: 10.1111/j.1432-1033.1973.tb02688.x